Programação Detalhada (Laboratorial) do IICCBB School of Science

28 de novembro de 2012 - 15:07

 

Lab Classes

 

 

Title

Faculty

TA

Lab

Program

Program

1.               

The hippocampal cell culture and neuronal polarity (Thu and Fri)

Alfredo Caceres

Rodrigues, F. A. R; Marques, M.M.M.; Rodrigues, P.A.

LOE/UFC

LNC/UFC

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Culturing hippocampal neurons

2.               

Sperm cryopreservation in powdered coconut water

Salgueiro, (ram); Nunes, (goat); Salmito-Vanderley, (fish); Silva, (dogs); Lavor, (poltry)

Santos, Bárbara Mara

NIB/UECE

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Local: Núcleo Integrado de Biotecnologia/UECE.

2. Sperm cryopreservation in powdered coconut water

– Ram: Methods of sperm collection. Sperm evaluation. Products and protocols for sperm cryopreservation;

– Goat: Methods of sperm collection. Sperm evaluation. Products and protocols for sperm cryopreservation;

– Fish: Methods of sperm collection. Sperm evaluation. Products and protocols for sperm cryopreservation;

– Dog: Methods of sperm collection. Sperm evaluation. Products and protocols for sperm cryopreservation;

– Poutry: Methods of sperm collection. Sperm evaluation. Products and protocols for sperm cryopreservation.

3.               

Non-Targeting analyses as a tool to study metabolism

Cameron, Bassini, Moreira & Waters

Moreira’s lab

LAP/UNIFOR

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Teorias de analise proteômicas em geral

4.               

Practical of the molecular biology  and Gene expression analysis using microarrays

 

Sotelo-Silveira

Vasconcelos, Fábio R.

Franklin, Maria Lucia T.

Malta, Daniele

LBH/UECE

UNIFOR

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basic aspects of evaluating mRNA diferential expression using microrarrays. The practical activity will be learning how to analyze microarray data (generated by the researcher or available in public databases) and the basic concepts to do RNA-seq (RNA diferential expression studies using deep sequencing). 

 

 

5.               

Discussion of the experimental bases to demonstrate macromolecular Cell to Cell transfer (Mon and Tue)

Sotelo

Franklin, Maria Lucia T.

Malta, Daniele

LBH/UECE

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Discussion of the experimental bases to demonstrate macromolecular Cell to Cell transfer and molecular

6.               

Stable isotope applications to measure human metabolic processes/ Mass Spectrometry

Peter Watt

Selene Maia de Morais e Ícaro Gusmão Pinto Vieira

PADETEC/UFC

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The course aims identify the interactions of physiological and biochemical factors that induce fatigue. Muscle function will be examined using interventions known to affect or elicit fatigue. The practical course will examine the roles of exercise-elevated metabolites (e.g. ammonia) or environmental interventions (e.g. hypoxia) on fatigue, alone or in combinations to measure the importance of each towards fatigue processes. Experimental protocol to measuring central voluntary and evoked muscle function using a range of techniques including mass spectrometry will be showed.

 

 


 

Theoretical Courses

 

 

Title

Faculty

 

 

Program

7.               

Searching biomarkers by proteomics

Javier Ambrosio

 

 

1.     Differences between genomics, transcriptomics and proteomics.

2.     Proteins, proteome and proteomics.

3.     Experimental strategies for studying proteomics.

4.     Detection of postransductional modifications of cytoskeletal proteins.

5.     Concepts of bioinformatics and retrieval of information from web sites.

6.     Detection of proteins by proteomics in different biological fluids

7.     Detection of proteins by proteomics of cells processed under different conditions as treatment with drugs, chemicals and biomaterials. Microscopia confocal.]

8.               

Metabolic adaptations to physiopathological conditions. Exercise; Diabetes and Cancer.

Wagner Coelho

Teoria

 

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9.               

Actin cytoskeleton modulates calcium signaling during maturation and fertilization of starfish oocytes

Luigia Santella

Teoria

 

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